如何解决 TiffFileError: Not a Tiff File and Byte Problem with KeyError: b'\x89P'

问题描述

我正在建立一个计算机视觉项目来检测和处理 GFP 蛋白.我不断收到关于我的文件不是 Tiff 图像和字节错误的错误.我不太明白他们的意思，也没有在网上找到任何关于它的信息.

I am setting up a computer vision project to detect and process GFP proteins. I keep getting errors about my file not being a Tiff Image and a Byte Error. I don't quite understand what they mean and haven't found anything about it online.

我已经确保文件路径正确，并尝试将文件更改为 Tiff 格式.现在在 Finder 上，它说它是 TIFF 图像，但仍然给出错误.

I have already made sure that the file path is correct and have tried changing the file into Tiff Format. Now on Finder, it says that it is a TIFF Image but still gives an error.

import tifffile
from colicoords import Data, Cell, CellPlot
import matplotlib.pyplot as plt


binary_img = tifffile.imread('organoid_images/gfp/cells1.tif')
data = Data()
data.add_data(binary_img, 'binary')
cell = Cell(data)
cell.optimize()
cp = CellPlot(cell)

plt.figure()
cp.imshow('flu_514', cmap='viridis', interpolation='nearest')
cp.plot_outline()
cp.plot_midline()
plt.show()

错误信息:

Traceback (most recent call last):
  File "/Users/CosmoCrash/opencvblobs/lib/python3.7/site-packages/tifffile/tifffile.py", line 2236, in __init__
    byteorder = {b'II': '<', b'MM': '>'}[header[:2]]
KeyError: b'\x89P'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "gfp.py", line 6, in <module>
    binary_img = tifffile.imread('organoid_images/gfp/cells1.tif')
  File "/Users/CosmoCrash/opencvblobs/lib/python3.7/site-packages/tifffile/tifffile.py", line 715, in imread
    with TiffFile(files, **kwargs_file) as tif:
  File "/Users/CosmoCrash/opencvblobs/lib/python3.7/site-packages/tifffile/tifffile.py", line 2238, in __init__
    raise TiffFileError('not a TIFF file')
tifffile.tifffile.TiffFileError: not a TIFF file

推荐答案

您的文件以 \x89P 开头是 PNG 文件，而不是 TIFF，因为这是一个 PNG 签名，而 TIFF 文件以 II 开头(如果是 Intel 顺序)，或者 MM 如果是 Motorola 顺序.

Your file starting \x89P is a PNG file, not TIFF, as that is a PNG signature, whereas TIFF files start II if in Intel order, or MM if in Motorola order.

如果在 Linux/macOS 上，请尝试运行:

If on Linux/macOS, try running:

file cells1.tif

请参阅 维基百科，了解 Warren 建议的 PNG 签名说明.

See Wikipedia for description of PNG signature, as suggested by Warren.

请参阅 维基百科了解 TIFF 标头.

See Wikipedia for description of TIFF header.

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