1、IGV的网址:http://software.broadinstitute.org/software/igv/(java环境)
常见的几种输入格式bam/sam(比对文件) TDF(bam的精简版) bed(注释文件) gtf/gff(注释文件)
PSL(blat比对结果) VCF(snp,indel的信息) WIG(UCSC数据库推荐格式wiggle track format) IGV(IGV默认的格式)
2、操作指南:http://www.docin.com/p-1847147664.html
1、导入参考基因组: genomes >> load genome from file >> reference.fasta
2、导入注释文件: file >> load from file >> reference.gtf #文件要按坐标排序,已经排过
3、bam格式转TDF格式: rools >> runigvtools >> count >> sampleA.bam >> run ,再生成另外一个sampleB.bam文件的TDF
bam文件必须和参考基因组文件同一目录下,否则失败
4、导入TDF文件: file >> load from file >>sampleA.bam.tdf ;file >> load from file >>sampleB.bam.tdf
5、导入甲基化文件(IGV格式):file >> load from file >> sampleA.igv ;file >> load from file >> sampleB.igv